Determination of aromatic retinoic acid ethyl ester in aromatic retinoic acid ethyl ester capsules by UV-1700SPC ultraviolet spectrophotometry

Determination of Aromatic Retinoic Acid Ethyl Ester in Aromatic Retinoic Acid Ethyl Ester Capsules by UV-1700SPC Ultraviolet Spectrophotometry

Key words: ultraviolet spectrophotometry; aromatic retinoic acid ethyl ester; aesthetic analyzer ; UV-1700SPC abstract: determination of aromatic retinoic acid ethyl ester capsules in the content of aromatic retinoic acid ethyl ester. The wavelength was (306 ± 1) nm by ultraviolet spectrophotometry. RESULTS: The linear relationship between the concentration of aromatic retinoic acid ethyl ester and the absorbance was good in the range of 2～12 μg/mL. The regression curve of the standard curve was A=0.06893C-0.005333, r=0.9999, the average recovery was 99.9%, RSD=0.74. %. Conclusion: UV spectrophotometry is simple, accurate and reproducible, and can be used for quality control of aromatic retinoic acid ethyl ester. Arotinoid ethylester is a representative of the third-generation retinoids. It is a potent, long-acting differentiation-inducing agent for the treatment of severe psoriasis, keratosis, and rheumatism. , immune rickets, malignant tumors, leukemia, etc. The content determination of the preparation has not been reported yet. The content of the aromatic retinoic acid ethyl ester is determined by ultraviolet spectrophotometry, and the operation is simple and rapid, and the result is satisfactory. 1 Instrument and reagent UV-1700SPC UV spectrophotometer; electronic balance. Aromatic retinoid reference substance and aromatic retinoid capsule; anhydrous ethanol (analytical grade); excipient (medicinal). 2 Methods and Results 2.1 UV absorption spectrum Weigh about 10mg of aromatic retinoic acid ethyl ester reference substance, dissolved in absolute ethanol and diluted into 10μg / mL solution, scanning in the wavelength range of 200 ~ 400nm, the results at 306nm wavelength The maximum absorption, the maximum absorption peak of the aromatic retinoic acid ethyl ester capsule solution is exactly the same as that of the reference substance, and the capsule and the additive have no absorption at this wavelength, so 306nm is selected as the measurement wavelength. 2.2 Drawing of the standard curve accurately weighed 10 mg of aromatic retinoic acid ethyl ester dried in a desiccator for 24 h, dissolved in absolute ethanol and made up to 100 mL, and shaken. Accurately measure 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mL, place in a 25 mL volumetric flask, add anhydrous ethanol to the scale, and shake. The absorbance was measured at a wavelength of (306 ± 1) nm. The results showed that the linear relationship between the concentration of aromatic retinoic acid ethyl ester and the absorbance was good in the range of 2 - l2 μg / mL. The regression equation was A=0.06893C-0.005333, r=0.9999. 2.3 Reproducibility test Three samples (batch number: 030415) were taken and the content was determined according to the method of 2.6. The results were 0.0154, 0.0158, 0.0152 mg/particle, and RSD=0.25%. 2.4 Recovery rate test Weigh accurately the amount of dried aromatic retinoic acid reference substance, weigh the starch and other auxiliary materials according to the proportion of the prescription, fully grind and mix evenly, accurately weigh the simulated capsule powder about 300 mg, respectively In 6 25mL volumetric flasks, add 20mL of absolute ethanol, fully shaken to completely dissolve the aromatic retinoic acid ethyl ester, add absolute ethanol to the mark, shake well, filter with dry filter paper, discard the primary filtrate, and take the filtrate. Absorbance was measured at (306 ± 1) nm with anhydrous ethanol as a blank, and the content and recovery were calculated by substituting the regression equation. 2.5 Stability test Take the test solution under 2.4, store it in the dark at room temperature, and measure the absorbance at 0, 1, 2, 4, 8, 16, 24h. The results showed that the absorbance of this product was basically unchanged within 24 hours. 2.6 Determination of sample content Take 20 tablets of this product, accurately pour out the contents, mix well and then accurately weigh the appropriate amount (about 100μg of aromatic retinoic acid ethyl ester), place it in a 25mL volumetric flask, add absolute ethanol 20mL, shake to completely dissolve the aromatic retinoic acid ethyl ester, add absolute ethanol to the mark, shake. Absorbance was measured at (306 ± 1) nm with anhydrous ethanol as a blank, and the content was calculated by substituting the regression equation. Key words: ultraviolet spectrophotometry; aromatic retinoic acid ethyl ester; US analytical instrument ; UV-1700SPC